Table 1.
Antibody | Species/Clonality | Source (Catalogue Number) | Dilution |
---|---|---|---|
Anti-BDNF | Rabbit/Polyclonal | Santa Cruz, sc-546 | 1:500 |
Anti-TrkB | Mouse/Monoclonal | BD Bioscience, 610101 | 1:5000 |
Anti-SYT-I | Mouse/Monoclonal | Stressgen, SYA-130 | 1:5000 |
Anti-SYT-IV | Rabbit/Polyclonal | Santa Cruz, sc-30095 | 1:1000 |
Anti-SNAP-25 | Rabbit/Polyclonal | Stressgen, ADI-VAM-SV012 | 1:5000 |
Anti-PKCζ | Goat/Polyclonal | Santa Cruz, sc-216-G | 1:1000 |
Anti-pCamKII | Rabbit/Monoclonal | Cell Signaling Tech, 12716 | 1:1000 |
Anti-CamKII | Rabbit/Monoclonal | Cell Signaling Tech, 11945 | 1:1000 |
Anti-pErk1/2 | Rabbit/ Monoclonal | Cell Signaling Tech, 4370 | 1:10,000 |
Anti-Erk1/2 | Rabbit/Monoclonal | Cell Signaling Tech, 4695 | 1:10,000 |
Anti-GluR1 | Rabbit/Monoclonal | Merck-Millipore, 04-855 | 1:5000 |
pp-38 | Rabbit/Monoclonal | Cell Signaling Tech, 4511 | 1:1000 |
p-38 | Rabbit/Monoclonal | Cell Signaling Tech, 8690 | 1:1000 |
pGSK3α/β (Y) | Mouse/Monoclonal | Merck-Millipore, 05-413 | 1:1000 |
pGSK3α/β (S) | Rabbit/Monoclonal | Cell Signaling Tech, 9327 | 1:1000 |
GSK3α/β | Rabbit/Monoclonal | Cell Signaling Tech, 5676 | 1:1000 |
Anti-β-actin | Mouse/Polyclonal | Sigma-Aldrich, A2228 | 1:10,000 |
BDNF: brain-derived neurotrophic factor; TrkB: tyrosine receptor kinase B; SYT: synaptotagmin; SNAP-25: synaptosomal-associated protein-25; PKC: protein kinase C; CamKII: Ca2+/calmodulin-dependent protein kinase II; Erk1/2: extracellular regulated protein kinase 1/2; GluR1: glutamate receptor 1; p38: p38 mitogen-activated protein kinase; GSK3α/β: glycogen synthase kinase 3-α/β.