Table 2.
Pathways determined from integration of metabolomic and proteomic data of SpD treated AC16 cells. Identified proteins and metabolites were analyzed using Integrated Molecular Pathway Level Analysis (IMPaLA) for pathway enrichment.
| Pathway | No. of Overlapping Genes | No. of Genes in Pathway | No. of Overlapping Metabolites | No. of Metabolites in Pathway | p-Value | q-Value |
|---|---|---|---|---|---|---|
| Glutathione metabolism | 12 | 54 (54) | 2 | 38 (38) | 8 × 10−6 | 4 × 10−4 |
| Protein digestion and absorption | 12 | 90 (90) | 3 | 47 (47) | 3 × 10−5 | 9 × 10−4 |
| Gap junction | 16 | 88 (88) | 1 | 11 (11) | 7 × 10−5 | 0.002 |
| Sulfur metabolism | 4 | 10 (10) | 2 | 33 (33) | 8 × 10−5 | 0.002 |
| Aminoacyl-tRNA biosynthesis | 12 | 66 (66) | 2 | 52 (52) | 9 × 10−5 | 0.002 |
| Choline metabolism in cancer | 12 | 99 (99) | 2 | 11 (11) | 1 × 10−4 | 0.002 |
| Central carbon metabolism in cancer | 11 | 65 (65) | 2 | 37 (37) | 1 × 10−4 | 0.003 |
| Long-term depression | 9 | 60 (60) | 1 | 9 (9) | 0.003 | 0.033 |
| Long-term potentiation | 9 | 67 (67) | 1 | 7 (7) | 0.004 | 0.043 |