Figure 2.

High Resolution Clear Native Electrophoresis (HrCNE) (left and middle) and two-dimensional polyacrylamide gel electrophoresis (2D-SDS-PAGE) (right) analysis of E. tenella complex II. For HrCNE gels, 10 μg and 27 μg per lane of bovine heart mitochondria and E. tenella mitochondria-rich fraction were loaded, respectively. Left and middle panels were stained by SDH activity and CBB staining, respectively. The red arrow indicates the location of SDH stained bands of 745 kDa in the CBB stained gel, which was cut off and loaded into 2D-SDS-PAGE gel (right) and stained by CBB. A total of 15 bands, named according to their calculated molecular weight, were stoichiometrically stained by CBB (right).