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. 2019 Jan 9;8(1):32. doi: 10.3390/cells8010032

Figure 3.

Figure 3

ADAM15 siRNA inhibits EC chemotaxis and EC tube formation. (A) Knockdown of ADAM15 was confirmed by Western blotting. (B) ADAM15 contributes to RA synovial fluid-mediated HUVEC chemotaxis. RA synovial fluid was depleted of rheumatoid factor. HUVECs were treated with control or ADAM10 siRNA. (n = number of different RA synovial fluid samples used). (C and D) RA synovial fluids were used to stimulate HUVEC tube formation on Matrigel. Photographs were taken at 100×. Representative photographs of non-treated HUVECs (C) and ADAM15 siRNA-treated HUVECs (D). RA synovial fluid-stimulated HUVECs treated with ADAM15 siRNA formed significantly fewer tubes on Matrigel than non-treated HUVECs (n = number of patients). For both chemotaxis and tube formed assays, means are presented with SEM, * p < 0.05 was significant; (hpfs, high-power fields.).