Figure 3.

SP-SA-E7-4-1BBL DNA vaccine shows therapeutic efficacy against TC-1 tumor with long-term immunological memory: (A) Tumor volume after subcutaneous injection of TC-1 cells in C57BL/6 mice. At days 3 and 10 after tumor challenge, mice were immunized on shaved abdominal skin with 1 µg of DNA using gene gun system. Tumor volumes are represented by mean ± SEM. (B) Survival graph after TC-1 cell challenge. Mice were euthanized when the tumor volume was higher than 1500 mm³. * p < 0.05, SP-SA-E7-4-1BBL compared with CRT-E7wt, SP-SA-4-1BBL and empty vector, ** p < 0.05, SP-SA-E7-4-1BBL compared with SP-SA-4-1BBL and empty vector. Two-way ANOVA and post-hoc Tukey’s test were performed.