Table 1.
Primers used to amplify inflammasome and complement components.
| Primer | Species | Gene | Orientation | Sequence (5′-3′) |
|---|---|---|---|---|
| 1 | Human | NLRP3 | F | ATTCGGAGATTGTGGTTGGG |
| R | AGGGCGTTGTCACTCAGGTC | |||
| 2 | pro-caspase-1 | F | CTCAGGCTCAGAAGGGAATGTC | |
| R | TGTGCGGCTTGACTTGTCC | |||
| 3 | pro-IL-1b | F | GCTCGCCAGTGAAATGATGG | |
| R | CAGAGGGCAGAGGTCCAGG | |||
| 4 | C3 | F | CACTATGATCCTTGAGATCTGTACCA | |
| R | GGAGCAAAGCCAGTCATCA | |||
| 5 | C3aR | F | GACATCCAGGTGCTGAAGCC | |
| R | ACTGGGGGCTCATTCCATG | |||
| 6 | C5aR1 | F | GCTGACCATACCCTCCTTCCT | |
| R | CCGTTTGTCGTGGCTGTAGTC | |||
| 7 | C5aR2 | F | TGCTGTTTGTCTCTGCCCATC | |
| R | GTCAGCAGGATGATGGAGGG | |||
| 8 | Mouse | pro-caspase-1 | F | AATGAAGTTGCTGCTGGAGGA |
| R | CAGAAGTCTTGTGCTCTGGGC | |||
| 9 | pro-IL-1b | F | TGGACCTTCCAGGATGAGGACA | |
| R | GTTCATCTCGGAGCCTGTAGTG |