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. 2018 Dec 26;11(1):20. doi: 10.3390/cancers11010020

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Radiation response of prostate cancer in an immunocompetent host. The radiosensitivity of prostate cancer (TRAMP-C1 and the corresponding CRPC cells), as demonstrated by (a) tumor growth delay of ectopic tumors after 15 Gy irradiation, and by (b) the orthotopic tumor model in immunocompetent mice. Representative images and quantitative data are shown 12 days after 15 Gy radiotherapy (RT) or sham irradiation. The y axis represents the relative ratio, normalized to the tumor size of TRAMP-C1 tumors in sham-irradiated mice (* p < 0.05). (c) Expression levels of Ki-67 and p-H2AX in tumors were evaluated by immunohistochemical staining (IHC) analysis. Scale bars: 20 μm. The y axis represents the ratio normalized by the value of TRAMP-C1 under control conditions. The recruitment of myeloid-derived suppressor cells (MDSCs) was evaluated by FACS using GrI-CD11b staining in the spleen (d). The MDSC level was the value of the number of CD11b+Gr1+ cells divided by the total cell number. The level of MDSCs in the tumors was evaluated by FACS (e) and by immunofluorescence using CD11b staining (f). Scale bars: 50 μm. The levels of interleukin (IL)-6 in the tumors were examined by immunofluorescence (IF) analysis (and in serum by ELISA with mice bearing tumors 48 h after 15 Gy RT or sham irradiation). (g). Columns: Means of three separate experiments; bars: SD; * p < 0.05.

Radiation response of prostate cancer in an immunocompetent host. The radiosensitivity of prostate cancer (TRAMP-C1 and the corresponding CRPC cells), as demonstrated by (a) tumor growth delay of ectopic tumors after 15 Gy irradiation, and by (b) the orthotopic tumor model in immunocompetent mice. Representative images and quantitative data are shown 12 days after 15 Gy radiotherapy (RT) or sham irradiation. The y axis represents the relative ratio, normalized to the tumor size of TRAMP-C1 tumors in sham-irradiated mice (* p < 0.05). (c) Expression levels of Ki-67 and p-H2AX in tumors were evaluated by immunohistochemical staining (IHC) analysis. Scale bars: 20 μm. The y axis represents the ratio normalized by the value of TRAMP-C1 under control conditions. The recruitment of myeloid-derived suppressor cells (MDSCs) was evaluated by FACS using GrI-CD11b staining in the spleen (d). The MDSC level was the value of the number of CD11b+Gr1+ cells divided by the total cell number. The level of MDSCs in the tumors was evaluated by FACS (e) and by immunofluorescence using CD11b staining (f). Scale bars: 50 μm. The levels of interleukin (IL)-6 in the tumors were examined by immunofluorescence (IF) analysis (and in serum by ELISA with mice bearing tumors 48 h after 15 Gy RT or sham irradiation). (g). Columns: Means of three separate experiments; bars: SD; * p < 0.05.