Figure 4.

Role of ADT in the radiation sensitivity of CRPC in immunocompetent mice. The role of ADT on the radiosensitivity of CRPC was demonstrated by (a) tumor growth delay of TRAMP-HR ectopic tumors after 15 Gy irradiation. We also show the representative images 12 days after 15 Gy irradiation with or without ADT and by (b) the orthotopic tumor model 12 days after 15 Gy irradiation in immunocompetent mice. Representative images and quantitative data are shown. The y axis represents the relative ratio, normalized to the tumor size of TRAMP-HR tumors in sham-irradiated male mice (* p < 0.05). (c) Expression levels of p-H2AX were evaluated by IHC in CRPC tumors with different types of treatment. Scale bars: 20 μm. (d) The recruitment of MDSCs in the spleen evaluated by FACS using GrI-CD11b staining. The quantification for MDSC levels was the calculation of the value of the number of CD11b+Gr1+ cells divided by the total cell number. Columns: Means of three separate experiments; bars: SD; * p < 0.05. (e) The extent of tumor-infiltrating T cells (TILs), shown by immunofluorescence analysis, of tumor specimens using CD3 staining in CRPC tumors with different types of treatment. Scale bars: 50 μm