Figure 2.

Antrocin and radiation synergistically arrest the cell cycle at G2/M and regulate apoptosis-related molecules in radioresistant PCa cells. (A) PC3-KD cells were treated with the vehicle control, IR (2 Gy) alone, antrocin (100 μM) alone, or IR combined with antrocin, and incubated for 48 h. Cell cycle distribution based on DNA content was analyzed through flow cytometry. (B) Different cell phases were plotted as the percentage of total events. (C,D) Cell lysates were harvested and subjected to Western blot analysis to determine the protein expression levels. The data represent one of three independent experiments. β-actin was used as a loading control.