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. 2019 Jan 9;11(1):63. doi: 10.3390/cancers11010063

Figure 5.

Figure 5

Reduction in LDOC1 expression promoted tumor growth in subcutaneous xenograft models of lung cancer. (A) Expression of LDOC1 mRNA (left) and protein (right) in A549-derived clones stably suppressed either LDOC1 expression (A549-shLDOC1-B11 and -D9) or knockdown controls (A549-shCtrl-D10 and-C9). ** p < 0.01. (B–E) A tumor xenograft experiment was performed by subcutaneously injecting A549-shLDOC1-D9 and A549-shCtrl-D10 cells into the right and left back of Balb/c nu/nu mice, respectively. (B) The volumes of xenograft tumors in the mice were measured as described in Materials and Methods. The individual (C) and the average (D) tumor weight (data indicated mean tumor weight ± SD, n = 10) and the images (E) of harvested tumors derived from A549-shLDOC1-D9 and A549-shCtrl-D10 cells on each mouse were compared on day 35. (F) Representative immunostaining of LDOC1, pJAK2, JAK2, pSTAT3Y705, and STAT3 in xenograft tumors derived from A549-shLDOC1-D9 and A549-shCtrl-D10 cells. Scale Bar, 50 μm.