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Double-stranded (ds) RNAs in isolate QT5-19 of Botrytis cinerea. The electrophoregram on the left was carried out in a 1.0% agarose gel after 1.5-h electrophoresis under room temperature. The electrophoregram on the right was carried out in a 0.7% agarose gel after 15-h electrophoresis at 4 °C. The dsRNAs were treated with RNase-free DNase I and S1 nuclease before electrophoresis. M = DL5000 dsDNA marker (TaKaRa).
