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. 2019 Jan 3;11(1):24. doi: 10.3390/v11010024

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Virus particles isolated from the mycelia of isolate QT5-19 of Botrytis cinerea. (A) Two transmission electron microscope (TEM) graphs showing the shape and size of the virus particles of the mycovirus in QT5-19; (B) Electrophoregrams showing the two dsRNAs extracted from the virus particles (VP) and the mycelia of QT5-19 (Mycelia). The electrophoregram on the right was created in a 1% agarose gel after 1.5-h electrophoresis under room temperature. The electrophoregram on the left was created in a 0.7% agarose gel after 15-h electrophoresis at 4 °C. M = DL5000 marker (TaKaRa); (C) An SDS-PAGE (10%) electrophoregram showing the band of the structural protein (coat protein) extracted from the virus particles of the mycovirus in QT5-19. The gel was stained with Coomassie brilliant blue R-250. M = PageRuler™ Prestained Protein Ladder.