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. 2019 Jan 3;11(1):24. doi: 10.3390/v11010024

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Horizontal transmission of BcPV2 through hyphal contact in pair cultures on PDA. (A) A pair culture on PDA with the colonies of isolate QT5-19 (BcPV2 donor, pink color) and a recipient (gray color). The symbol (*) in the colony indicated the area where a mycelial agar plug was removed and transferred to PDA for establishing a BcPV2-transmitted derivative; (B) Four PDA cultures (20 °C, 20 day) of the B. cinerea isolates free of infection by BcPV2 (BcPV2⁻); (C) Four PDA cultures (20 °C, 20 day) of the transfected derivatives of B. cinerea. BcPV2⁺, infected by BcPV2, BcPV2⁻, not infected by BcPV2.