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BRCA2 is a client protein of HSP90. (A) Immunoblot of BRO cells treated with or without 60 min of hyperthermia in the presence of cycloheximide (50 µg/mL), ganetespib (50 nM), or both. (B) Immunoblots of HeLa cells treated with or without hyperthermia in the presence of various combinations of proteasomal inhibitor MG132 (50 µM), autophagy inhibitor bafilomycin (10 nM), and HSP90-inhibitor ganetespib (50 nM).
