Figure 4.

Efforts to identify the E2-conjugating enzyme that mediates degradation of BRCA2 upon hyperthermia. (A) Results of individual knock-down of all known ubiquitin E2-conjugating enzymes in U2OS cells. Efficiencies of E2 siRNAs were previously assessed by quantitative polymerase chain reaction PCR [37]. (B) Results of simultaneous knock-downs of E2-conjugating enzymes belonging to specific families in U2OS cells. (C) Results of knock-down of the small ubiquitin-like modifier (SUMO)-E2 enzyme Ubiquitin Conjugating Enzyme 9 (UBC9) in U2OS cells.