Figure 7.

Oxidative stress as an inducer of BRCA2 degradation. (A) BRCA2 levels in BRO cells subjected to hyperthermia in the presence of indicated doses of antioxidant compounds N-acetylcysteine (NAC), ascorbic Acid (Asc), or dithiothreitol (DTT). (B) BRCA2 levels in BRO cells subjected to the indicated dose of ultraviolet-B and lysed three hours afterwards. (C) BRCA2 levels in BRO cells treated with various concentrations of oxidative stress-inducers H₂O₂, tert-Butyl hydroperoxide (t-BHP), and Rotenone. (D) BRCA2 levels in BRO cells exposed to hyperthermia or H₂O₂ (0.1 mM) in the presence or absence of proteasome inhibitor MG132 (50 µM).