Table 6.
List of antibodies.
| Host | Epitope | Dilutions Used | Reference/Suppliers |
|---|---|---|---|
| Mouse | BRCA2 (OP95) | WB 1:1000 | EMD Millipore |
| Mouse | FLAG (M2) | WB 1:1000–1:5000 | Sigma-Aldrich |
| Mouse | ORC2 (68348) | WB 1:1000 | Abcam |
| Rabbit | Brca2 (27976) | WB 1:500 | Abcam |
| Mouse | GFP (clones 7.1 and 13.1) | WB 1:1000–1:5000 | Sigma-Aldrich |
| Mouse | PARP-1 (C2-10) | WB 1:5000 | Enzo Lifesciences |
| Mouse | BRCA1 (OP92) | WB 1:250 | EMD Millipore |
| Rabbit | Cyclin A (C-19) | WB 1:5000 | Santa Cruz |
| Goat | RAD54 (D-18) | WB 1:1000 | Santa Cruz |
| Rabbit | RAD51 (2307) | WB 1:10,000 | Home-made [100] |
| Rabbit | RAD51 (2308) | IF 1:10,000 | Home-made [100] |
| Mouse | GRB2 | WB 1:1000 | BD Pharmingen |
| Mouse | HSP90 (AC88, 13492) | WB 1:5000 | Abcam |
| Rabbit | CDC37 (3618S) | WB 1:1000 | Cell Signaling |
| Goat | UBC9 (N-15) | WB 1:1000 | Santa Cruz |
| Rabbit | CHIP (PA1-015) | WB 1:1000 | Thermo Scientific |
| Sheep | Peroxidase anti-Mouse IgG (H+L) | WB 1:2000 | Jackson Immunoresearch |
| Donkey | Peroxidase anti-Rabbit IgG (H+L) | WB 1:2000 | Jackson Immunoresearch |
| Donkey | Peroxidase anti-Goat IgG (H+L) | WB 1:2000 | Jackson Immunoresearch |
| Goat | Alexa-Fluor-594 (red) | IF 1:1000 | Thermo Scientific |