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. 2018 Dec 21;11(1):12. doi: 10.3390/cancers11010012

Figure 4.

Figure 4

Figure 4

Figure 4

In vivo tumorigenicity of R2J cells after intracranial implantation in nude mice of (a) 2 × 105 cells cultivated in the monolayer (b) 2 × 105 or (c) 1000 cells cultivated in spheres. MRI acquisitions were performed post implantation at the times indicated. Mice were sacrificed after the last MRI. Tumor volumes were calculated by adding each tumor x slice thickness (0.5 mm²). (a) Implantation of 2 × 105 R2J monolayer cultivated cells. (b) Implantation of 2 × 105 R2J sphere cells. (c) Implantation of 1000 R2J sphere cells.