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. 2018 Dec 28;10(1):21. doi: 10.3390/genes10010021

Figure 5.

Figure 5

Identification of genes directly regulated by HNF4A during the endoderm to hepatic transition. (A) Diagram showing strategy used to identify genes that are direct targets of HNF4A with high confidence; (B) Expression profiles determined by RT-qPCR of genes that are direct targets of HNF4A during the transition of iPSC-derived endoderm to a hepatic fate. t-test compared expression levels at day eight vs. day seven; (C) Bar graphs showing RT-qPCR analyses of mRNAs expressed from HNF4A target genes in hepatic progenitor cells derived from control and HNF4A depleted iPSCs at day eight of differentiation. (D) Bar graphs showing results of ChIP-qPCR analyses of HNF4A occupancy in hepatic progenitor cells derived from control (dark grey) and HNF4A-depleted (light grey) iPSCs at day eight of differentiation. Error bars represent s.e.m, n = 3 independent differentiations. Student’s t-test, (*) p ≤ 0.05. (**) p ≤ 0.005.