Table 3.
Characterization of scFv 10FG2.
| (A) | |||||
| TOXIN | kon (1/Ms) × 105 | koff (1/s) × 10−5 | KD (M) × 10−9 | TR (min) | KD (M) scFv × 10−9 ER |
| Cll1 | 2.65 ± 0.65 | 6.3 ± 1.3 | 0.23 ± 0.005 | 264.5 | 1.3 |
| Cn2 | 1.50 ± 0.30 | 10.2 ± 0.8 | 0.70 ± 0.075 | 163.4 | 1.0 |
| Css2 | 1.95 ± 0.05 | 55.0 ± 5.0 | 2.75 ± 0.25 | 30.0 | ND |
| Cll2 | 2.20 ± 0.50 | 90.0 ± 10.0 | 4.20 ± 0.40 | 18.5 | 5.8 |
| CeII9 | 1.50 ± 0.10 | 92.5 ± 4.5 | 6.10 ± 0.10 | 18.0 | ND |
| Ct1a | 1.70 ± 0.81 | 100.0 ± 0.01 | 8.00 ± 4.00 | 16.7 | 29.0 |
| (B) | |||||
| Toxin | µg/20 g Mouse | Alive/Total | |||
| Control | Pre-Incubated Mix | ||||
| Cll1 | 1.7 | 1/8 | 8/8 | ||
| Cll2 | 1.5 | 0/8 | 8/8 | ||
| CeII9 | 3.0 | 0/8 | 8/8 | ||
| Ct1a | 2.0 | 0/8 | 8/8 | ||
(A) Kinetic constants of scFv 10FG2 against several toxins. Biosensor assays were performed at 25 °C at a flow rate 50 µL min−1. The constants were calculated using the Langmuir (1:1) model in the Bia-evaluation 3.1 software. In the right column, KD values for the scFv ER-1 [22]. ND: non determined. TR: time of residence. (B) Neutralization tests of scFv 10FG2 against Cll1, Cll2, CeII9, and Ct1a toxins. Mice were intraperitoneally injected with the toxin (controls) or with a preincubated mixture of toxin and antibody (experimental) at a molar ratio of 1:5 (toxin: scFv).