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. 2019 Jan 1;9(1):108–121.

Figure 3.

Figure 3

GAS5 is associated with miR-182. A. GAS5 mainly located in cytoplasm of SMMC-7721 cells shown by qRT-PCR. B. Regions of putative interaction between GAS5 and miR-182. C. Luciferase activity in SMMC-7721 cells cotransfected with miR-NC or miR-182 and luciferase reporters containing nothing, GAS5 or mutant GAS5. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. D. Luciferase activity in Hep3B cells cotransfected with miR-NC (inh-NC) or miR-182 inhibitor (inh-182) and luciferase reporters containing nothing or GAS5. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. E. MS2-RIP was performed to detect miR-182 endogenously interacted with GAS5 in SMMC-7721 and Hep3B cells. F. SMMC-7721 and Hep3B cell lysates were incubated with biotin-labeled GAS5; after pull-down, miR-182 was assessed by qRT-PCR. G. Anti-AGO2 RIP was performed in SMMC-7721 and Hep3B cells transiently overexpressing miR-NC or miR-182, followed by qRT-PCR to detect GAS5 associated with AGO2. H. The expression of miR-182 was detected by qRT-PCR in SMMC-7721 cells with overexpression of wild-type or mutant GAS5. I. The expression of miR-182 was detected by qRT-PCR in Hep3B cells with GAS5 knockdown. J. The expression of GAS5 was detected by qRT-PCR in SMMC-7721 and Hep3B cells transfected with miR-NC or miR-182. K. The expression of GAS5 was detected by qRT-PCR in SMMC-7721 and Hep3B cells transfected with miR-NC or miR-182 inhibitor. Bars represent mean ± SD. *p<0.05.