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. 2019 Jan 1;9(1):108–121.

Figure 4.

Figure 4

miR-182 directly targets ANGPTL1. A. Hep3B cells were transfected with miR-182. The miR-182 level was detected by qRT-PCR. B. Hep3B cells were transfected with miR-182 inhibitor. The miR-182 level was detected by qRT-PCR. C. The effect of miR-182 overexpression on migration and invasion in Hep3B cells was detected by transwell assay. Statistical results of the transwell assay (three independent experiments) was shown. D. The effect of miR-182 knockdown on migration and invasion in Hep3B cells was detected by transwell assay. Statistical results of the transwell assay (three independent experiments) was shown. E. Regions of putative interaction between ANGPTL1 3’UTR and miR-182. F. Luciferase activity in Hep3B cells cotransfected with miR-NC or miR-182 and luciferase reporters containing nothing, ANGPTL1 3’UTR or its mutant. G. ANGPTL1 mRNA levels in indicated Hep3B cells was detected by qRT-PCR. H. ANGPTL1 protein levels in indicated Hep3B cells was detected by western blot. Bars represent mean ± SD. *P<0.05.