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. 2019 Jan 1;9(1):108–121.

Figure 5.

Figure 5

GAS5 upregulates ANGPTL1 expression to suppress aggressiveness. A. miR-182 overexpression abolished the GAS5-mediated upregulation of ANGPTL1 mRNA level. B. miR-182 knockdown rescued GAS5 shRNA-mediated downregulation of ANGPTL1 mRNA level. C. Luciferase activity in stable Hep3B cell clones transfected with luciferase reporters containing ANGPTL1 3’UTR. D. Luciferase activity in stable SMMC-7721 cell clones transfected with luciferase reporters containing ANGPTL1 3’UTR. E. The expression of miR-182 and ANGPTL1 in 60 paired HCC tissues and adjacent non-tumor tissues was measured by qRT-PCR. The correlation between ANGPTL1 and GAS5 RNA levels in HCC tissues was then analyzed. F. The stable Hep3B cell clones were transfected with miR-182 or ANGPTL1 siRNA. After 48 hours, the migration and invasion was detected by transwell assay. G. The stable SMMC-7721 cell clones were transfected with inh-182 or ANGPTL1. After 48 hours, the migration and invasion was detected by transwell assay. Bars represent mean ± SD. *P<0.05.