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. 2019 Jan 1;9(1):134–144.

Figure 4.

Figure 4

ENPP1 knockdown reduce the EMT induced by TGF-β. A. Wound healing and migratory analysis of HCC827-shNS and HCC827-shENPP1 cell lines. Scale bar = 200 μm. B. Graph demonstrates the migratory ability of HCC827 and HCC827-shENPP1 cell lines (mean ± SD) from 3 separate experiments. C. HCC827-shNS and HCC827-shENPP1 cell lines were treated with TGF-β (5 ng/ml) for the indicated time periods, and expression of E-cadherin and vimentin was analyzed by immunoblot. D, E. HCC827 and HCC827-shENPP1 cell lines were treated with TGF-β (5 ng/ml) for 4 days. Expression of E-cadherin and vimentin, (green) was analyzed by immunofluorescence staining and DAPI (blue) were imaged by confocal microscopy. F. Wound healing and migratory analysis of HCC827-shNS and HCC827-shENPP1 cell lines treated with TGF-β (5 ng/ml) treatment for the indicated time periods. Scale bar = 200 μm.