Figure 4.

ACP5 promoted proliferation and invasion in CRC cells. (A) RT-qPCR and western blotting showed the ACP5 expression in five CRC cell lines. (B, C) ACP5 overexpression (B) or knockdown (C) efficiency was confirmed by RT-qPCR in CRC cells. (D, E) Effects of ACP5 overexpression (D) or knockdown (E) on proliferation was evaluated by CCK-8 assay. (F, G) Effects of ACP5 overexpression (F) or knockdown (G) on invasion was measured by transwell assay. Results shown are the mean ± SD (**P < 0.01, ***P < 0.001) of triplicate determination from three independent experiments.