ACP5 regulated FAK/PI3K/AKT signaling pathway in CRC cells. A. Expression of total-FAK, phospho-FAK, total-PI3K, phospho-PI3K, total-AKT, phospho-AKT proteins were determined in HT-29 and SW480 cells transfected with pcDNA3.1-ACP5. B. Expression of total-FAK, phospho-FAK, total-PI3K, phospho-PI3K, total-AKT, phospho-AKT proteins were determined in HCT116 and SW1116 cells transfected with siRNAs-ACP5. Results shown are the mean ± SD (**P < 0.01, ***P < 0.001) of triplicate determination from three independent experiments.