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. 2019 Jan 1;9(1):94–107.

Figure 3.

Figure 3

HIPK2 SIM mutants have dominant-negative function. A. U2OS cells were transfected with GFP-HIPK2, CFP-HIPK2 or a SIM-VK mutant and nuclear HA-PML-IV. Cells were fixed and immunostained 24 hrs after transfection. B. The HIPK2 R861W and N951I mutants have dominant-negative function. U2OS cells were transfected with GFP-HIPK2, Myc-HIPK2 or various mutants and nuclear HA-PML-IV. Cells were fixed and immunostained 24 hrs after transfection. Images were obtained with a confocal fluorescent microscope at an excitation wavelength of 488 nm and 543 nm, respectively.