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. 2019 Jan 1;9(1):94–107.

Figure 5.

Figure 5

HIPK2 SIM mutants impair co-localization between AML1b and PML-I. A. U2OS cells were transfected with HA-AML1b in the presence or absence of Flag-PML-I in combination with GFP-HIPK2 or GFP-HIPK2 SIM mutant. Cells were fixed and immunostained 24 hrs after transfection. B. U2OS cells were transfected with HA-AML1b and Flag-PML-I in combination with Myc-HIPK2 or Myc-HIPK2 mutants (HIPK2 SIM-VK, R861W or N951I). Cells were fixed and immunostained 24 hrs after transfection. Zoom image is 10 times magnification of inset. Arrows indicate the AML1b-containing puncta independent of PML-I. Images were obtained with a confocal fluorescent microscope at an excitation wavelength of 488 nm and 543 nm, respectively.