Figure 6.

HIPK2 SIM mutants impair AML1b-dependent differentiation of K562 cells. A. Morphological features of K562 cells in which differentiation was induced by PMA treatment for 24 hrs. K562 cells were transfected with GFP-HIPK2, or GFP-HIPK2 mutant (HIPK2 SIM-VK mutant, R861W or N951I). Cytospins were stained with Wright-Giemsa. Original amplification, ×400. Arrows indicate the differentiated K562 cells. B. The number of differentiated or undifferentiated K562 cells were counted, and are shown on the graph. The data were statistically analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test (**P < 0.01). Data are presented as the mean ± SEM.