miR-758 inhibits proliferation, colony formation, migration, and invasion and induces apoptosis of OS cells in vitro. A. HOS and U2OS cells were transfected with miR-758 mimics and miR-758 inhibitor, respectively. RT-qPCR was performed at 48 h post-transfection to measure miR-758 expression. *P < 0.05 vs. miR-NC. #P < 0.05 vs. NC inhibitor. B, C. The regulatory roles of miR-758 in OS cell proliferation and colony formation were evaluated using MTT and clonogenic survival assays, respectively. *P < 0.05 vs. miR-NC. #P < 0.05 vs. NC inhibitor. D. Flow cytometry analysis to determine the effect of miR-758 on OS cell apoptosis. *P < 0.05 vs. miR-NC. #P < 0.05 vs. NC inhibitor. E, F. Migration and invasion abilities of miR-758 mimics-transfected HOS and miR-758 inhibitor-transfected U2OS cells were determined using transwell assays. *P < 0.05 vs. miR-NC. #P < 0.05 vs. NC inhibitor.