Figure 6.

miR-758 inhibits the activation of the Wnt/β-catenin pathway in OS in vitro and in vivo. A. HOS cells were co-transfected with miR-758 mimics and pc-HMGA1 or pcDNA3.1. U2OS cells were co-transfected with miR-758 inhibitor and HMGA1 siRNA or NC siRNA. Western blot analysis was performed to detect p-β-catenin, β-catenin, and cyclin D1 protein levels 72 h after transfection. B. The protein levels of p-β-catenin, β-catenin and cyclin D1 in OS tissues was detected through western blot analysis.