Figure 7.

miR-758 inhibits OS tumor growth in vivo. HOS cells were transfected with miR-758 mimics or miR-NC. The transfected cells were collected and subcutaneously injected into the flanks of nude mice after 24 h. A. Representative images of the tumor xenografts in miR-758 mimics and miR-NC groups. B. After 2 weeks, the volume of the tumor xenografts was detected and calculated using the following formula: Volume (mm³) = Width² (mm²) * Length (mm)/2. *P < 0.05 vs. miR-NC. C. Nude mice were sacrificed one month after the inoculation. The tumor xenografts were excised and weighed. *P < 0.05 vs. miR-NC. D. Total RNA was isolated from xenografts derived from miR-758 mimics- or miR-NC-transfected HOS cells, and miR-758 expression was determined. *P < 0.05 vs. miR-NC. E. Western blot analysis was performed to determine HMGA1, p-β-catenin, β-catenin, and cyclin D1 protein levels in the xenografts.