Figure 5.
Effect of PACAP-38 on the phosphorylation of Akt and PTEN in C6 cells. Effect of the H89 PKA inhibitor on PACAP638 induced inhibition of PTEN phosphorylation. (A–C) Cells were treated with 10−7 M PACAP-38 for the indicated times. Total cell lysates were analyzed by immunoblotting (WB) with antibodies recognizing total Akt, phosphorylated Akt, phosphorylated PTEN or GAPDH. (A) corresponds to a representative WB experiment. (B,C) Cumulative data from three independent experiments after quantification by densitometry. Data were normalized by attributing a value of 1 for the control experiment. (D,E) Cells were treated for 3 h with 10−7 M PACAP-38, in the presence or not of 1 µM H89. (D) corresponds to a representative WB experiment. (E) Cumulative data from three independent experiments after quantification by densitometry. Quantification data are the mean ± SD. Data were analyzed using the Kruskal–Wallis test and Dunn’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001, ns: not significant).