Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 4;11(1):18. doi: 10.3390/toxins11010018

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of DON and FB₁, alone or in association, and IP6 on apoptosis (Ccasp-3) and cell proliferation (Ki-67) in jejunal explants. Explants exposed to control treatment (□); IP6 5 mM (); deoxynivalenol (DON) (); fumonisin B₁ (FB₁) (); DON + FB₁ (■); DON + IP6 (); FB₁ + IP6 (); DON + FB₁ + IP6 (). (A) Mean number of Ccasp-3 immunostained cells per field on explants exposed to different treatments. Mean values with different superscript letters were significantly different (p ≤ 0.05). (B) Control treatment: mild Ccasp-3 cytoplasmic immunostaining in crypt cells. (C) DON 10 µM + FB₁ 70 µM: diffuse Ccasp-3 cytoplasmic immunostaining in crypt cells. (D) DON 10 µM + FB₁ 70 µM + IP6 5 mM: decrease in Ccasp-3 cytoplasmic immunostaining in crypt cells. (E) Mean number of Ki-67 immunostained cells per field on explants exposed to different treatments. Mean values with different superscript letters were significantly different (p ≤ 0.05). (F) Control treatment: mild Ki-67 nuclear immunostaining in crypt cells. (G) DON 10 µM + FB₁ 70 µM: diffuse Ki-67 nuclear immunostaining in crypt cells. (H) DON 10 µM + FB₁ 70 µM + IP6 5 mM: decrease in Ki-67 nuclear immunostaining in crypt cells. (B–D; F–H: immunoperoxidase method, bar 25 µm).

Inline graphic — Effects of DON and FB₁, alone or in association, and IP6 on apoptosis (Ccasp-3) and cell proliferation (Ki-67) in jejunal explants. Explants exposed to control treatment (□); IP6 5 mM (); deoxynivalenol (DON) (); fumonisin B₁ (FB₁) (); DON + FB₁ (■); DON + IP6 (); FB₁ + IP6 (); DON + FB₁ + IP6 (). (A) Mean number of Ccasp-3 immunostained cells per field on explants exposed to different treatments. Mean values with different superscript letters were significantly different (p ≤ 0.05). (B) Control treatment: mild Ccasp-3 cytoplasmic immunostaining in crypt cells. (C) DON 10 µM + FB₁ 70 µM: diffuse Ccasp-3 cytoplasmic immunostaining in crypt cells. (D) DON 10 µM + FB₁ 70 µM + IP6 5 mM: decrease in Ccasp-3 cytoplasmic immunostaining in crypt cells. (E) Mean number of Ki-67 immunostained cells per field on explants exposed to different treatments. Mean values with different superscript letters were significantly different (p ≤ 0.05). (F) Control treatment: mild Ki-67 nuclear immunostaining in crypt cells. (G) DON 10 µM + FB₁ 70 µM: diffuse Ki-67 nuclear immunostaining in crypt cells. (H) DON 10 µM + FB₁ 70 µM + IP6 5 mM: decrease in Ki-67 nuclear immunostaining in crypt cells. (B–D; F–H: immunoperoxidase method, bar 25 µm).