Table 3.

Primer sequences of some genes for RT-PCR.

Gene	Primer Sequence (5′-3′)	Accession Number	Size (bp)
GAPDH	F: ATGACCACAGTCCATGCCATC	XM-004387206.1	271
	R: CCTGCTTCACCACCTTCTTG
Cell apoptosis genes
Bcl-2	F: AGAGCCGTTTCGTCCCTTTC	XM-003122573.2	270
	R: GCACGTTTCCTAGCGAGCAT
Bax	F: ATGATCGCAGCCGTGGACACG	XM-003355975.1	296
	R: ACGAAGATGGTCACCGTCTGC
Caspase-3	F:TTGGACTGTGGGATTGAGACG	NM-214131.1	165
	R: CGCTGCACAAAGTGACTGGA
Cytokines gene
IL-6	F: GCTCTCTGTGAGGCTGCAGTTC	NM_213867.1	107
	R: AAGGTGTGGAATGCGTATTTATGC
Barrier function genes
ZO-1	F: CCTGAGTTTGATAGTGGCGTTGA	XM-003353439.2	269
	R: AAATAGATTTCCTGCCCAATTCC
Occludin	F: ACCCAGCAACGACATA	NM_001163647.2	155
	R: TCACGATAACGAGCATA
Nutrient transporter genes
SGLT1	F: TCATCATCGTCCTGGTCGTCTC	M34044.1	144
	R: CTTCTGGGGCTTCTTGAATGTC
GLUT2	F: ATTGTCACAGGCATTCTTGTTAGTCA	NM_001097417.1	273
	R: TTCACTTGATGCTTCTTCCCTTTC
PepT1	F: CAGACTTCGACCACAACGGA	NM_214347.1	99
	R: TTATCCCGCCAGTACCCAGA
ASCT2	F: CTGGTCTCCTGGATCATGTGG	DQ231578.1	172
	R: CAGGAAGCGGTAGGGGTTTT