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. 2019 Jan 8;8(1):16. doi: 10.3390/antiox8010016

Figure 1.

Figure 1

Cytosolic ICDH1 activity. ICDH (Isocitrate dehydrogenase) activity was measured for 800 ng of ICDH by monitoring the change in absorbance at 340 nm due to the isocitrate-dependent rate of NADP+ reduction at 25 °C in 1 mL reaction medium containing 5 mM MgCl2, 250 μM of NADP+ and (A) different concentrations (0–5 mM) of DL-isocitric acid or (B) different concentrations (0–1 mM) of NADP in 100 mM phosphate buffer (KOH, pH 7.5). (C) 2.5 mM DL-isocitric acid in 100 mM phosphate buffer at different pH (6–8.5). Error bars represent SE (Standard Error) (n = 3).