Figure 6.
ICDH activity in planta. (A) ICDH activity was measured for 40 µg of protein extracts at 25 °C by monitoring the change in absorbance at 340 nm due to the isocitrate-dependent rate of NADP+ reduction. Wild-type (Col-0) or mutant A. thaliana plants were grown in soil in a controlled growth chamber (180 µE m−2 s−1, 16 h day/8 h night, 22 °C 55% RH day, 20 °C 60% RH night) for 2 weeks. (B) Same design as in (A). In Col-O+GSNO and Col-0+SNP, protein extracts of wild-type plants were treated with 1 mM GSNO or SNP for 30 min before performing activity tests. Error bars represent SE (n = 3). * p < 0.01, ** p < 0.001 for statistical differences compared to non-treated samples (Student’s t test).