Skip to main content
. 2019 Jan 10;11(1):46. doi: 10.3390/v11010046

Figure 2.

Figure 2

The role of the actin cytoskeleton in spherical IAV assembly in host cells. (A) Contrasting roles for the actin cytoskeleton in spherical IAV assembly in different cell types. In IAV-permissive cells, such as epithelial cell lines and a macrophage-like cell line, the actin cytoskeleton either promotes or has no effects on IAV particle production. In contrast, the actin cytoskeleton restricts HA-M2 association and spherical IAV assembly in primary human macrophages. (B) Proposed mechanisms by which actin restricts spherical IAV assembly in primary human macrophages. Microdomain segregation: F-actin partitions HA- and M2-enriched plasma membrane microdomains. Suppression of membrane curvature: F-actin restricts HA-mediated curvature induction at the plasma membrane, which is required for M2 recruitment to the assembling particle. Blocking of cytoplasmic components: F-actin restricts trafficking and incorporation of other curvature-inducing structural components, that is, M1 and vRNP, to virus assembly sites.