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. 2019 Jan 11;11(1):50. doi: 10.3390/v11010050

Figure 5.

Figure 5

SDS-PAGE analysis of the trypsinisation products of the purified gp053 polysheaths. (A) Lanes: M—molecular mass marker, Page RulerTM Prestained Protein Ladder (Thermofisher), 1—recombinant gp053, 2–4 recombinant gp053 incubated with trypsin (the digestion time is indicated under the lanes). (B) Lanes: M—molecular mass marker, Page RulerTM Prestained Protein Ladder (Thermofisher); 1—recombinant gp053 incubated for 24 hours in TE buffer; 2—recombinant gp053 incubated for 24 hours in TE buffer with trypsin. The arrows indicate the full-length gp053 (1,3) and its fragments (2,4–6), excised from the gel for LC–MS/MS analysis.