Table 1.

Half maximal inhibitory concentration (IC₅₀) values and standard errors for CRC cell lines after incubation with afatinib for 72 h under normoxic and hypoxic conditions.

IC50 Afatinib 72 h (μM)
Cell Line	Cetuximab Resistance Status	Normoxia (21% O2)	Hypoxia (1% O2)
Lim1215	Sensitive	0.081 ± 0.021	0.178 ± 0.055
CaCo2	Sensitive	0.341 ± 0.199	0.604 ± 0.307
SW48	Intrinsically resistant	2.379 ± 0.869	2.109 ± 0.691
HT29	Intrinsically resistant	1.805 ± 0.041	1.816 ± 0.117
Lim1215-PBS	PBS-treated control, sensitive	0.007 ± 0.002	0.010 ± 0.003
Lim1215-R	Acquired resistance	0.174 ± 0.030	0.458 ± 0.060
CaCo2-PBS	PBS-treated control, sensitive	0.591 ± 0.384	0.260 ± 0.197
CaCo2-R	Acquired resistance	1.570 ± 0.264	1.398 ± 0.270