Table 1.
Information on the primers and predeveloped assays used for qPCR.
| MyHC Isoform | Gene | Primer Sequence (5′→ 3′) | Amplicon Length |
| embryonal | MyHCembry | F: CGGGTCCTTCCCATCTGA R: GCAGCAGCCGTGAGAAATC P: FAM-CTGCCCGGCTTTGGTC |
75 |
| I | MyHC-I | F: CGTGCTCCGTCTTCTTTCCTT R: GAAGAAAGAGGCTCAAGCTGGAA P: FAM-CTGCTCTCAGGCCCC |
60 |
| 2a | MyHC-2a | F: GTCCTGCTTTAAAAAGCTCCAAGAA R: AGAGGTCCCTCTTAGCAAGTGA P: FAM-CAGGCTGCATCTTC |
73 |
| 2b | MyHC-2b | F: AACACTTTAAGTAGTTGTCTGCCTTGA R: AGGGCACTAGATGTACCTCTTATGT P: FAM-CCTGCCACCGTCTTC |
76 |
| 2x | MyHC-2x | F: GCTTCAAGTTCTGCCCCACTT R: TGGCAGCCCAGTCAAAGAC P: FAM-CTTTGAGATGCAACCTTG |
59 |
| Full Gene Name | Gene | Amplicon Length | Assay ID |
| Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha | PGC-1α | 96 | Ss03393114_u1 |
| Peroxisome proliferator-activated receptor gamma | PPARγ | 72 | Ss03394829_m1 |
| Lipoprotein-lipase | LPL | 66 | Ss03394612_m1 |
| Carnitine palmitoyltransferase 1B | CPT1B | 60 | Ss03378792_u1 |
| Eukaryotic ribosomal (r) 18S rRNA | 18S rRNA | 69 | Hs03003631_g1 |
F = forward primer; R = reverse primer; P = probe.