Figure 2.

In vitro complex between 5′UTR nt 1–290 and NS5B nt 8374–8676 RNAs. ³²P-labeled RNA probe (20 nM) was incubated with increased amounts of unlabeled RNA target (50–800 nM) in low magnesium binding buffer. Complexes were separated in a native 5% acrylamide gel buffered with TBM. Plots of % probe complex versus target concentration ± SE were derived from three separate experiments. See Materials and Methods Section 2.5 RNA Binding Assays for derivation of K_d and B_max values.