Table 2.

HCV RNA levels and progeny virus titers in Huh-7 cells transfected with 5′UTR and NS5B mutant genome constructs.

Virus Construct	HCV RNA Copies 1	MT WT	Progeny Virus 1	MT WT	Progeny HCV RNA Copies 2	MT WT
WT genome	1.7 × 106 ± 3.5 × 105		6.9 × 103 ± 2.1 × 103		1.9 × 104 ± 3.8 × 103
5′UTRLM	3.2 × 105 ± 1.2 × 105	−5.3	1.4 × 103 ± 7.6 × 102	−4.8	6.3 × 103 ± 1.8 × 103	−2.9
NS5BLM	2.9 × 105 ± 1.1 × 105	−6.0	2.1 × 103 ± 1.0 × 103	−3.2	1.4 × 103 ± 1.4 × 102	−13.1
5′UTRSM	6.7 × 105 ± 2.9 × 105	−2.6	6.9 × 102 ± 2.4 × 102	−10	4.4 × 103 ± 1.3 × 103	−4.3
NS5BSM	4.0 × 105 ± 1.6 × 105	−4.2	9.9 × 102 ± 4.0 × 102	−7	3.3 × 103 ± 5.9 × 102	−5.7
DblLM	4.7 × 106 ± 2.9 × 106	1.9	7.2 × 103 ± 1.9 × 103	2	2.8 × 104 ± 5.3 × 103	1.7
DblSM	4.1 × 106 ± 2.7 × 106	1.7	9.4 × 103 ± 2.3 × 104	1.7	5.4 × 104 ± 2.2 × 104	1.3

¹ Focus-forming units (FFU)/mL ± SE; ² HCV genome copies/1 μg total RNA ± SE.