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. 2019 Jan 21;11(1):209. doi: 10.3390/nu11010209

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental protocol. Each participant took part in two overfeeding periods according to a randomized, cross-over design. WM: weight maintenance diet, LP-HF: hypercaloric (150% energy requirement high-sucrose, low protein-high fat); HP-LF: hypercaloric (150% energy requirement high-sucrose, high protein-low fat); MRS: magnetic resonance spectroscopy for measurement of IHCL and IMCL; M. test: metabolic test, consisting of measurements of energy expenditure, plasma hormones, and substrate concentrations after ingestion of WM meal providing 40% of total energy requirements (D0), or LP-HF/HP-LF meals providing 60% of total energy requirements.