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. 2019 Jan 1;8(1):17. doi: 10.3390/cells8010017

Figure 8.

Figure 8

Immunofluorescence staining of integrin β3, F-actin, and paxillin in BMMs. Murine BMMs were treated with control shRNA/S1PR2 shRNA for one day or treated with vehicle/ JTE013 for 30 min. Then, cells were cultured in media containing M-CSF (50 ng/mL) alone or M-CSF with RANKL (250 ng/mL) for two days. On the third day, the media was changed with or without RANKL and/or JTE013. Some of the cells were co-cultured with both RANKL and Aa-stimulated media (Aa-media, 200 µL/mL) for another day. (A,C) show representative images of integrin β3, F-actin, and DAPI staining in BMMs (four to six cells per image). (B,D) show representative images of paxillin, F-actin, and DAPI staining in BMMs (four to six cells per image). (E,H) show cell fluorescence intensity of F-actin. (F,I) show cell fluorescence intensity of integrin β3. (G,J) show cell fluorescence intensity of paxillin. (* p < 0.05, ** p < 0.01, *** p < 0.001).