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. 2018 Dec 20;11(1):5. doi: 10.3390/cancers11010005

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glioblastoma, IDH wild type. (a) CD34+ endothelial cells of arterioles and capillaries in direct contact with Nestin+ tumor cells; double immunostaining with CD34 (DAB) and Nestin (Fast Red), original magnification (OM) ×400. (b) Vessel co-option. Sleeve of tumor cells around capillaries; CD34, DAB, OM ×400. (c) Id., with several Nestin+ tumor cells; DAB, OM ×200. (d) Mild infiltration. Reactive astrocytes on small vessels; GFAP, DAB, OM ×200; (e) More intense infiltration. Reactive astrocytes on vessels; GFAP, DAB, OM ×200; (f) High infiltration. Reactive astrocytes with end-feet on small vessels; GFAP, DAB, OM ×400. IDH, isocitrate dehydrogenase; DAB, 3,3′-Diaminobenzidine.