Table 2.
List of antibodies used for quantification of specific protein expression (WB), ICC, Co-IP, and ChIP assays.
| Antibody | Catalog No. | Conc. Ratio (WB) | Conc. Ratio (ICC) | Conc. Ratio (Co-IP) | Conc. Ratio (CHIP) |
|---|---|---|---|---|---|
| Primary Ab. | |||||
| Anti-KRT19 | SC-53258 | 1:1000 | - | - | - |
| Anti-Actin | SC-1616 | 1:1000 | - | - | - |
| Anti-β-catenin | SC-7199 | 1:2000 | 1:200 | 1:50 | 1:50 |
| Anti-RAC1 | SC-217 | 1:500 | 1:200 | 1:50 | 1:50 |
| Anti-c-Jun | SC-1694 | 1:1000 | - | - | - |
| Anti-p-SMAD3 | 9520S | 1:1000 | - | - | - |
| Anti-SMAD3 | 9513S | 1:1000 | - | - | - |
| Normal mouse IgG | SC-2025 | - | - | 1:50 | - |
| Normal rabbit IgG | SC-2027 | - | - | 1:50 | 1:50 |
| Second Ab. | |||||
| Anti-goat | SC-2020 | 1:1000 | - | - | - |
| Anti-rabbit | SC-2004 | 1:1000 | - | - | - |
| Anti-mouse | SC-2005 | 1:1000 | - | - | - |
| Alexa Fluor® 546 (goat anti-rabbit) | A11010 | - | 1:1000 | - | - |