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Influence of pH on influenza virus binding to bLf by ELISA. Virus samples incubated with buffers at different pH (from 7.4 to 4.0) were absorbed to bLf-coated plastic surfaces. The interaction between the different influenza virus samples and bLf was analyzed by an enzyme-linked immunosorbent assay, and results were expressed as the percentage of virus binding to bLf. Error bars represent the mean of three independent experiments. * p < 0.05.
