Fig. 2.

Gene editing of qPE9–1 led to small grains in the Nipponbare background. a Protein sequences of qPE9–1 in the NIP, qpe9–1#1, qpe9–1#2, and qpe9–1#3 plants. The GGL domain is shown by red boxes. b Structure of the qPE9–1 protein in NIP, qpe9–1#1, qpe9–1#2, and qpe9–1#3 plants. The numbers represent the different lengths of the truncated qPE9–1 protein in wild-type plants and the three mutants. c Grains of wild-type plants and the three qpe9–1 mutants. Bar = 1 cm. d Comparison of grain length between NIP and the three qpe9–1 mutants. e Comparison of 1000-grain weight between NIP and the three qpe9–1 mutants. Data are shown as means ± SDs (n = 15). Student’s t-test: ** P < 0.01