Table 2.
Differentially expressed proteins in root tissues of pokkali w.r.t IR64
| Protein identity | % coverage | Peptides | POK/IR64 | pvalue | MSU ID | Protein function |
|---|---|---|---|---|---|---|
| (A) Control UP | ||||||
| Os10g0191300 protein | 62.5 | 43 | 2.208 | 0.0038 | LOC_Os10g11500.1 | ‘stress.biotic’ |
| Phenylalanine ammonia-lyase | 37.4 | 32 | 1.5417 | 0.012 | LOC_Os02g41630.2 | ‘secondary metabolism.phenylpropanoids.lignin biosynthesis.PAL’ |
| Class III peroxidase 86 | 48.2 | 21 | 1.977 | 0.0032 | LOC_Os06g35520.1 | ‘misc.peroxidases’ |
| Putative r40c1 protein-rice | 41.6 | 14 | 1.8707 | 0.0498 | LOC_Os03g21040.2 | Stress responsive protein |
| Os03g0712700 protein | 28.2 | 10 | 2.5823 | 0.0167 | LOC_Os03g50480.1 | ‘not assigned.unknown’ |
| Malate dehydrogenase | 41.6 | 10 | 17.2187 | 0.0011 | LOC_Os10g33800.1 | ‘TCA / org. Transformation.other organic acid transformaitons.cyt MDH’ |
| Methylmalonate semi-aldehyde dehydrogenase | 23.6 | 11 | 2.0137 | 0.0305 | LOC_Os07g09060.1 | ‘amino acid metabolism.degradation.branched-chain group.valine’ |
| UTP--glucose-1-phosphate uridylyltransferase, putative | 30.5 | 10 | 5.5976 | 0.0071 | LOC_Os09g38030.1 | biological process |
| Beta-1,3-glucanase (Fragment) | 30.4 | 10 | 2.2909 | 0.0103 | LOC_Os01g51570.1 | ‘misc.beta 1,3 glucan hydrolases.glucan endo-1,3-beta-glucosidase’ |
| peroxidase precursor, putative, | 24 | 7 | 5.5976 | 0.0038 | LOC_Os05g06970.1 | ‘misc.peroxidases’ |
| |inhibitor I family protein, putative | 76.1 | 5 | 1.0471 | 0.0056 | LOC_Os01g42860.1 | ‘not assigned.unknown’ |
| |leucine aminopeptidase, chloroplast precursor | 10 | 4 | 3.6644 | 0.0376 | LOC_Os02g55140.1 | protein.degradation’ |
| 6-phosphogluconate dehydrogenase, decarboxylating | 20.4 | 4 | 2.1086 | 0.0291 | LOC_Os06g02144.1 | ‘OPP.oxidative PP.6-phosphogluconate dehydrogenase’ |
| succinyl-CoA ligase beta-chain, mitochondrial precursor | 14.7 | 3 | 3.0761 | 0.0114 | LOC_Os02g40830.1 | ‘TCA / org. Transformation.TCA.succinyl-CoA ligase’ |
| Os02g0582900 protein | 37.2 | 8 | 16.293 | 0.0177 | LOC_Os02g0582900 | NOT |
| Adenine phosphoribosyltransferase 1, putative, | 22.1 | 3 | 99.0832 | 0.0004 | LOC_Os12g39860.1 | ‘nucleotide metabolism.salvage.phosphoribosyltransferases.aprt’ |
| Superoxide dismutase | 3.0479 | 0.0509 | LOC_Os05g25850.1 | redox.dismutases and catalases’ | ||
| Putative isomerase | 15.3 | 2 | 22.9087 | 0.0085 | LOC_Os03g61330.2 | ‘amino acid metabolism.degradation.aromatic aa.tyrosine’ |
| Proteasome subunit alpha type | 22.4 | 2 | 1.888 | 0.031 | LOC_Os11g40140.1 | ‘protein.degradation.ubiquitin.proteasom’ |
| Sucrose synthase 2 | 4.5 | 1 | 2.3768 | 0.0195 | LOC_Os06g09450.3 | ‘major CHO metabolism.degradation.sucrose.Susy’ |
| 60 kDa chaperonin | 9.2 | 1 | 71.1214 | 0.0188 | LOC_Os10g32550.1 | ‘protein.folding’ |
| Os02g0583700 protein/hypothetical protein | 37.7 | 5 | 38.7258 | 0.0255 | LOC_Os02g37250.1 | ‘not assigned.unknown’ |
| Malic enzyme (Fragment) OS=Oryza sativa subsp. japonica GN=Os01g0723400 PE = 3 SV = 1 | 26.2 | 11 | 2.5351 | 0.0012 | LOC_Os01g52500.5 | ‘TCA / org. Transformation.other organic acid transformaitons.malic’ |
| Aldehyde dehydrogenase OS=Oryza sativa GN = Aldh PE = 2 SV = 1 | 20.8 | 7 | 3.4674 | 0.0314 | LOC_Os06g15990.1 | fermentation.aldehyde dehydrogenase’ |
| Os02g0196800 protein OS=Oryza sativa subsp. japonica GN=OJ1524_D08.17 PE = 2 SV = 1 | 16.3 | 2 | 7.656 | 0.038 | LOC_Os02g10310.1 | ‘amino acid metabolism.degradation.aromatic aa.tyrosine’ |
| Putative chaperonin 21 OS=Oryza sativa subsp. japonica GN=B1172G12.2 PE = 3 SV = 1 | 30.1 | 2 | 1.6144 | 0.0382 | LOC_Os06g09688.1 | ‘protein.folding’ |
| Class III peroxidase 70 OS=Oryza sativa subsp. japonica GN = prx70 PE = 3 SV = 1 | 16.7 | 3 | 99.0832 | 0.0187 | LOC_Os05g04490.1 | ‘misc.peroxidases’ |
| endo-1,3;1,4-beta-D-glucanase precursor | 15.5 | 1 | 8.2414 | 0.0397 | LOC_Os05g33100.1 | response to abiotic stimulus |
| Class III peroxidase 122 OS=Oryza sativa subsp. japonica GN=OJ1118_B06.10 PE = 3 SV = 1 | 24.2 | 1 | 8.9536 | 0.0364 | LOC_Os09g29490.1 | ‘misc.peroxidases’ |
| MPI, putative OS=Oryza sativa subsp. japonica GN = LOC_Os12g36220 PE = 4 SV = 2 | 28.8 | 2 | 87.9023 | 0.0168 | LOC_Os12g36220.1 | negative regulation of endopeptidase activity, response to stress |
| similar to oxygen evolving enhancer protein 3 domain containing protein, Ferredoxin-NADP reductase binding protein | 12 | 1 | 54.9541 | 0.0184 | LOC_Os07g36080.1 | protein modification process |
| Putative L-asparaginase OS=Oryza sativa subsp. japonica GN=OSJNBa0087M10.11 PE = 4 SV = 1 | 7.1 | 1 | 99.0832 | 0.0175 | LOC_Os03g40070.1 | ‘amino acid metabolism.degradation.aspartate family.asparagine.L-asparaginase’ |
| (B) Control Down | ||||||
| Acidic PR-1 type pathogenesis-related protein PR-1a OS=Oryza sativa subsp. japonica GN=PR-1a PE = 2 SV = 1 | 46.4 | 7 | 0.5702 | 0.0429 | LOC_Os07g03710.1 | ‘stress.biotic’ |
| Os08g0162800 protein OS=Oryza sativa subsp. japonica GN=P0577B11.140 PE = 4 SV = 1 | 65.9 | 9 | 0.2679 | 0.0035 | LOC_Os08g06550.1 | ‘lipid metabolism.FA synthesis and FA elongation.acyl-CoA binding protein’ |
| Ascorbate peroxidase OS=Oryza sativa subsp. japonica GN=Os07g0694700 PE = 2 SV = 1 | 37.1 | 5 | 0.4246 | 0.028 | LOC_Os07g49400.1 | ‘redox.ascorbate and glutathione.ascorbate’ |
| Triosephosphate isomerase OS=Oryza sativa subsp. japonica GN=P0569E11.2–1 PE = 3 SV = 1 | 16.8 | 4 | 0.0855 | 0.0233 | LOC_Os09g36450.1 | ‘PS.calvin cycle.TPI’ |
| peroxidase precursor, | 26.5 | 7 | 0.1406 | 0.0115 | LOC_Os04g59190.1 | ‘misc.peroxidases’ |
| fructose-bisphospate aldolase isozyme | 10.7 | 2 | 0.1127 | 0.0165 | LOC_Os11g07020.1 | carbohydrate metabolic process |
| Ubiquitin family domain containing protein | 23.1 | 3 | 0.6252 | 0.0184 | LOC_Os02g10510.1 | ‘protein.degradation.ubiquitin.ubiquitin’ |
| RNA recognition motif containing protein | 15.3 | 1 | 0.0111 | 0.0487 | LOC_Os01g68790.2 | ‘RNA.RNA binding’ |
| (C) Stress UP | ||||||
| Os01g0326000/ peroxidase | 26.3 | 6 | 23.1206 | 0.0005 | LOC_Os01g22230.1 | 'misc.peroxidases' |
| triosephosphate isomerase cytosolic | 14.5 | 2 | 2.3335 | 0.0502 | LOC_Os01g05490.1 | 'PS.calvin cycle.TPI' |
| calreticulin precursor protein | 25.7 | 6 | 4.1305 | 0.0682 | LOC_Os07g14270.3 | 'signalling.calcium' |
| Os02g0167300/tubulin beta-5 chain | 7.8 | 3 | 2.6792 | 0.0341 | LOC_Os02g07060.1 | 'cell.organisation' |
| (D) Stress Down | ||||||
| 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, 5-metH | 19.3 | 4 | 0.5248 | 0.0053 | LOC_Os12g42876.1 | 'amino acid metabolism.synthesis.aspartate family.methionine' |
| BBTI4 - Bowman-Birk type bran trypsin inhibitor precursor | 19.5 | 2 | 0.2938 | 0.0454 | LOC_Os01g03340.1 | NO |
| retrotransposon protein, putative, Ty1-copia subclas | 31.6 | 2 | 0.3251 | 0.0044 | LOC_Os08g03520.1 | 'stress.abiotic.cold' |
| thiol protease SEN102 precursor | 19 | 4 | 0.4831 | 0.0067 | LOC_Os09g39070.1 | 'protein.degradation.cysteine protease' |